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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Adenosine Monophosphate Activated Protein ELISA kit

E02A0043 Rat Adenosine Monophosphate Activated Protein ELISA kit

Rat Adenosine Monophosphate Activated Protein ELISA kit is suitable for the detection of samples from Rat species. Adenosine Monophosphate Activated Protein can also be called MPK, HAMPKb, 5'-AMP-Activated Protein Kinase Subunit Beta-1 Non-Catalytic Subunit, AMAP.

Specifications of Rat Adenosine Monophosphate Activated Protein ELISA kit

Product Information

Cat. No.

E02A0028

Product Name

Rat Adenosine Monophosphate Activated Protein ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000ng/ml

Sensitivity

1.0 ng/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AMPK ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AMPK. Standards or samples are then added to the microtiter plate wells and AMPK if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AMPK present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AMPK are added to each well to "sandwich" the AMPK immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AMPK and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMPK concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Adenosine Monophosphate Activated Protein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

93-112%

Linearity

Diluent Ratio

Range %

1:2

91-102

1:4

93-105

1:8

90-109

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AMPK and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Adenosine Monophosphate Activated Protein ELISA kit

Summary of the Assay Procedure for Rat Adenosine Monophosphate Activated Protein ELISA kit

Citations of Rat Adenosine Monophosphate Activated Protein ELISA kit

E02A0043 has been referenced in the below publications:

The preliminary study on the effects of AMPK activation induced by curcumin and aerobic exercise on middle-aged rats' skeletal muscle cellautophagy.

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