E02A0002 Rat Advanced Glycosylation End Products ELISA kit
Rat Advanced Glycosylation End Products ELISA kit is suitable for the detection of samples from Rat species. Advanced Glycosylation End Products can also be called Advanced Glycation End Products, AGE.
Product Information | |
Cat. No. | E02A0002 |
Product Name | Rat Advanced Glycosylation End Products ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000pg/ml |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AGEs ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AGEs antibody and an AGEs-HRP conjugate. The assay sample and buffer are incubated together with AGEs-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGEs concentration since AGEs from samples and AGEs-HRP conjugate compete for the anti-AGEs antibody binding site. Since the number of sites is limited, as more sites are occupied by AGEs from the sample, fewer sites are left to bind AGEs-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGEs concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-110% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-102 | |
1:4 | 94-107 | |
1:8 | 89-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AGEs and analogues was observed. | |
E02A0002 has been referenced in the below publications:
The role of exercising in improving the myocardial fibrosis of diabetic rats and its possible mechanism.
Impact and metabolism of exercise on collagen deposition in artery of diabetic rats.
Tempol ameliorates polycystic ovary syndrome through attenuating intestinal oxidative stress and modulating of gut microbiota composition-serum metabolites interaction.
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