En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Advanced Glycosylation End Products ELISA kit (E02A0002)

E02A0002 Rat Advanced Glycosylation End Products ELISA kit

Rat Advanced Glycosylation End Products ELISA kit is suitable for the detection of samples from Rat species. Advanced Glycosylation End Products can also be called Advanced Glycation End Products, AGE.

Products

Specifications of Rat Advanced Glycosylation End Products ELISA kit

Product Information

Cat. No.

E02A0002

Product Name

Rat Advanced Glycosylation End Products ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000pg/ml

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AGEs ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AGEs antibody and an AGEs-HRP conjugate. The assay sample and buffer are incubated together with AGEs-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGEs concentration since AGEs from samples and AGEs-HRP conjugate compete for the anti-AGEs antibody binding site. Since the number of sites is limited, as more sites are occupied by AGEs from the sample, fewer sites are left to bind AGEs-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGEs concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Advanced Glycosylation End Products ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-110%

Linearity

Diluent Ratio

Range %

1:2

93-102

1:4

94-107

1:8

89-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AGEs and analogues was observed.



BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Advanced Glycosylation End Products ELISA kit

Summary of the Assay Procedure for Rat Advanced Glycosylation End Products ELISA kit

Citations of Rat Advanced Glycosylation End Products ELISA kit

E02A0002 has been referenced in the below publications:

The role of exercising in improving the myocardial fibrosis of diabetic rats and its possible mechanism.

Impact and metabolism of exercise on collagen deposition in artery of diabetic rats.

Tempol ameliorates polycystic ovary syndrome through attenuating intestinal oxidative stress and modulating of gut microbiota composition-serum metabolites interaction.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.