Mouse Artemin ELISA kit is suitable for the detection of samples from mouse species. Artemin can also be called as NBN; EVN; ENOVIN; Neublastin; Neurotrophic Factor.
Specifications Of E03A0032 Mouse ART ELISA Kit
Porduct's Information | |
Cat.NO. | E03A0032 |
Porduct's Name | Mouse Artemin ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ART ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ART antibody and an ART-HRP conjugate. The assay sample and buffer are incubated together with ART-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ART concentration since ART from samples and ART-HRP conjugate compete for the anti-ART antibody binding site. Since the number of sites is limited, as more sites are occupied by ART from the sample, fewer sites are left to bind ART-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ART concentration in each sample is interpolated from this standard curve. |
Quality Control On Mouse ART ELISA Kit
CV | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery% | 95-106 | |
Linearity | Diluent ratio | Range % |
1:2 | 92-113 | |
1:4 | 97-105 | |
1:8 | 86-101 | |
Specificity/ Cross-reactivity | No significant cross-reactivity or interference between ART and analogues was observed. | |
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Summary Of The Assay Procedures For E03A0032 Mouse ART ELISA Kit
Citations Of Mouse Artemin ELISA Kit
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Product: E01A0032 Human Artemin ELISA kit;
