BlueGene E03A0050 Mouse Amyloid β Protein 42 ELISA Kit

BlueGene Biotech Mouse Amyloid β Protein 42 ELISA kit

E03A0050 Mouse Amyloid β Protein 42 ELISA kit

Mouse Amyloid β Protein 42 ELISA kit is suitable for the detection of samples from mouse species. Amyloid β Protein 42 can also be called beta Amyloid(1-42), beta Amyloid 1-42, beta-Amyloid 1-42, Amyloid 1-42, beta-Amyloid 42, A4, AAA, ABETA, ABPP, AD1, Alzheimers Disease Amyloid Protein, Amyloid B, Amyloid Beta A4 Protein Precursor.

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Specifications of Mouse Amyloid β Protein 42 ELISA kit

Product Information
Cat. No.	E03A0050
Product Name	Mouse Amyloid β Protein 42 ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	50-1000 pg/mL
Sensitivity	1.0 pg/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	50 pg/mL	1 vial
STANDARD C (0.5mL)	100 pg/mL	1 vial
STANDARD D (0.5mL)	250 pg/mL	1 vial
STANDARD E (0.5mL)	500 pg/mL	1 vial
STANDARD F (0.5mL)	1000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

Aβ42 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Aβ42 antibody and an Aβ42-HRP conjugate. The assay sample and buffer are incubated together with Aβ42-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Aβ42 concentration since Aβ42 from samples and Aβ42-HRP conjugate compete for the anti-Aβ42 antibody binding site. Since the number of sites is limited, as more sites are occupied by Aβ42 from the sample, fewer sites are left to bind Aβ42-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Aβ42 concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Amyloid β Protein 42 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	94-110%
Linearity	Diluent Ratio	Range %
	1:2	96-101
	1:4	92-113
	1:8	91-106
Specificity/Cross-reactivity	No significant cross-reactivity or interference between Aβ42 and analogues was observed.

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Summary of the Assay Procedure for Mouse Amyloid β Protein 42 ELISA kit

Citations of Mouse Amyloid β Protein 42 ELISA kit

E03A0050 has been referenced in the below publications:

Chronic noise exposure exacerbates AD-like neuropathology in SAMP8 mice in relation to Wnt signalling in the PFC and hippocampus.

Transcriptome analysis of the hippocampus in environmental noise-exposed SAMP8 mice reveals regulatory pathways associated with Alzheimer's disease neuropathology.

Environmental noise stress disturbs commensal microbiota homeostasis and induces oxi-inflammation and AD-like neuropathology through epithelial barrier disruption in the EOAD mouse model.