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E03A0050 Mouse Amyloid β Protein 42 ELISA Kit

Mouse Amyloid β Protein 42 ELISA kit is suitable for the detection of samples from mouse species. Amyloid β Protein 42 can also be called as beta Amyloid(1-42); beta Amyloid 1-42; beta-Amyloid 1-42; Amyloid 1-42; beta-Amyloid 42; A4; AAA; ABETA; ABPP; AD1; Alzheimers Disease Amyloid Protein; Amyloid B; Amyloid Beta A4 Protein Precursor.

Specifications Of E03A0050 Mouse Aβ42 ELISA Kit

Porduct's Information

Cat.NO.

E03A0050

Porduct's Name

Mouse Amyloid β Protein 42 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Aβ42 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Aβ42 antibody and an Aβ42-HRP conjugate. The assay sample and buffer are incubated together with Aβ42-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Aβ42 concentration since Aβ42 from samples and Aβ42-HRP conjugate compete for the anti-Aβ42 antibody binding site. Since the number of sites is limited, as more sites are occupied by Aβ42 from the sample, fewer sites are left to bind Aβ42-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Aβ42 concentration in each sample is interpolated from this standard curve.


Quality Control On Mouse Aβ42 ELISA Kit

CV

Intra Variation% <10%

Inter Variation% <12%

Recovery%

94-110

Linearity

Diluent ratio

Range %

1:2

96-101

1:4

92-113

1:8

91-106

Specificity/

Cross-reactivity

No significant cross-reactivity or interference between Aβ42 and analogues was observed.


BlueGene Biotech Product Show

Summary Of The Assay Procedures For E03A0050 Mouse Aβ42 ELISA Kit

Summary Of The Assay Procedures For E03A0050 Mouse Aβ42 ELISA Kit

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