E03T0018 Mouse Triglyceride ELISA kit
The Mouse Triglyceride ELISA kit can be used to identify samples from the mouse species. Triglyceride can also be called TAG, Triacylglycerol, Triacylglyceride, TG.
Specifications of Mouse Triglyceride ELISA kit
Product Information | |
Cat. No. | E03T0018 |
Product Name | Mouse Triglyceride ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000ug/ml |
Sensitivity | 1.0ug/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/ml | 1 vial |
STANDARD B (0.5mL) | 50 ug/ml | 1 vial |
STANDARD C (0.5mL) | 100 ug/ml | 1 vial |
STANDARD D (0.5mL) | 250 ug/ml | 1 vial |
STANDARD E (0.5mL) | 500 ug/ml | 1 vial |
STANDARD F (0.5mL) | 1000 ug/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TG ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TG antibody and an TG-HRP conjugate. The assay sample and buffer are incubated together with TG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TG concentration since TG from samples and TG-HRP conjugate compete for the anti-TG antibody binding site. Since the number of sites is limited, as more sites are occupied by TG from the sample, fewer sites are left to bind TG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TG concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Triglyceride ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TG and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Triglyceride ELISA kit
Citations of Mouse Triglyceride ELISA kit
E03T0018 has been referenced in the below publications：
Oxamate Improves Glycemic Control and Insulin Sensitivity via Inhibition of TissueLactate Production in db/db Mice.
The research on antioxidant effect and active function of Tyr-Ala di-peptide.
种具有降血脂降血糖活性的组合物及其制备方法与应用。
