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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit

E03I0074 Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit

The Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit can be used to identify samples from the mouse species. Insulin Like Growth Factor Binding Protein 3 can also be called IGFBP-3, IGFBP3, BP-53, IBP3, insulin like growth factor binding protein 3.

Specifications of Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit

Product Information

Cat. No.

E03I0074

Product Name

Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

0.5-10ng/ml

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml1 vial

STANDARD B (0.5mL)

0.5 ng/ml

1 vial

STANDARD C (0.5mL)

1.0 ng/ml

1 vial

STANDARD D (0.5mL)

2.5 ng/ml

1 vial

STANDARD E (0.5mL)

5.0ng/ml

1 vial

STANDARD F (0.5mL)

10 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IGFBP3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IGFBP3 antibody and a IGFBP3-HRP conjugate. The assay sample and buffer are incubated together with IGFBP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGFBP3 concentration since IGFBP3 from samples and IGFBP3-HRP conjugate compete for the anti-IGFBP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGFBP3 from the sample, fewer sites are left to bind IGFBP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGFBP3 concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IGFBP3 and analogues was observed.


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Summary of the Assay Procedure for Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit

Summary of the Assay Procedure for Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit

Citations of Mouse Insulin Like Growth Factor Binding Protein 3 ELISA kit

E03I0074 has been referenced in the below publications:

Tetrahydroxystilbene glucoside extends mouse life span via upregulating neural klotho and downregulating neural insulin or insulin-like growth factor 1.


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