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BlueGene Biotech Mouse Total Tumor Necrosis Factor ELISA kit

E03T0034 Mouse Total Tumor Necrosis Factor ELISA kit

The Mouse Total Tumor Necrosis Factor ELISA kit can be used to identify samples from the mouse species. Total Tumor Necrosis Factor can also be called TNF, DIF, TNF-alpha, TNFA, TNFSF2, Tumour necrosis factor, TNF-伪, tumor necrosis factor, TNLG1F, Tumor necrosis factor alpha.

Specifications of Mouse Total Tumor Necrosis Factor ELISA kit

Product Information

Cat. No.

E03T0034

Product Name

Mouse Total Tumor Necrosis Factor ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000pg/ml

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/ml1 vial

STANDARD B (0.5mL)

50 pg/ml

1 vial

STANDARD C (0.5mL)

100 pg/ml

1 vial

STANDARD D (0.5mL)

250 pg/ml

1 vial

STANDARD E (0.5mL)

500 pg/ml

1 vial

STANDARD F (0.5mL)

1000 pg/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TNF ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TNF antibody and an TNF-HRP conjugate. The assay sample and buffer are incubated together with TNF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNF concentration since TNF from samples and TNF-HRP conjugate compete for the anti-TNF antibody binding site. Since the number of sites is limited, as more sites are occupied by TNF from the sample, fewer sites are left to bind TNF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNF concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Total Tumor Necrosis Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TNF and analogues was observed.


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Summary of the Assay Procedure for Mouse Total Tumor Necrosis Factor ELISA kit

Summary of the Assay Procedure for Mouse Total Tumor Necrosis Factor ELISA kit

Citations of Mouse Total Tumor Necrosis Factor ELISA kit

E03T0034 has been referenced in the below publications:

The study of biocompatibility of pristine and functionalized multi-walled carbon nanotubes.

Environmental noise stress disturbs commensal microbiota homeostasis and induces oxi-inflammmation and AD-like neuropathology through epithelial barrier disruption in the EOAD mouse model.

Short-and long-term toxicities of multi-walled carbon nanotubes in vivo and in vitro.


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