E03P0047 Mouse Procollagen Type III ELISA kit
The Mouse Procollagen Type III ELISA kit can be used to identify samples from the mouse species. Procollagen Type III can also be called PCOL-III, PC-III, PC3, Type III Procollagen.
Specifications of Mouse Procollagen Type III ELISA kit
Product Information | |
Cat. No. | E03P0047 |
Product Name | Mouse Procollagen Type III ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100ng/ml |
Sensitivity | 1.0ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 10 ng/ml | 1 vial |
STANDARD D (0.5mL) | 25 ng/ml | 1 vial |
STANDARD E (0.5mL) | 50 ng/ml | 1 vial |
STANDARD F (0.5mL) | 100 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PC3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PC3. Standards or samples are then added to the microtiter plate wells and PC3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PC3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PC3 are added to each well to “sandwich” the PC3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PC3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PC3 concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Procollagen Type III ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PC3 selectin and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Procollagen Type III ELISA kit
Citations of Mouse Procollagen Type III ELISA kit
E03P0047 has been referenced in the below publications：
Isolation, Purification and Hepatoprotective Effect of Polysaccharides from Dicliptera chinensis.
