Mouse Secretoglobin, Family 1A, Member 1/Clara Cell Specific Protein CC16 ELISA kit

BlueGene Biotech Mouse Secretoglobin, Family 1A, Member 1/Clara Cell Specific Protein CC16 ELISA kit

E03U0019 Mouse Secretoglobin, Family 1A, Member 1/Clara Cell Specific Protein CC16 ELISA kit

The Mouse Secretoglobin, Family 1A, Member 1/Clara Cell Specific Protein CC16 ELISA kit can be used to identify samples from the mouse species. Secretoglobin, Family 1A, Member 1/Clara Cell Specific Protein CC16 can also be called SCGB1A1, CC10, CC16, CCPBP, CCSP, UGB, UP-1, UP1, secretoglobin family 1A member 1.

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Specifications of Mouse Secretoglobin, Family 1A, Member 1/Clara Cell Specific Protein CC16 ELISA kit

Product Information
Cat. No.	E03U0019
Product Name	Mouse Secretoglobin, Family 1A, Member 1/Clara Cell Specific Protein CC16 ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	50-1000pg/ml
Sensitivity	1.0pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/ml	1 vial
STANDARD B (0.5mL)	50 pg/ml	1 vial
STANDARD C (0.5mL)	100 pg/ml	1 vial
STANDARD D (0.5mL)	250 pg/ml	1 vial
STANDARD E (0.5mL)	500 pg/ml	1 vial
STANDARD F (0.5mL)	1000 pg/ml	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

SCGB1A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SCGB1A1 antibody and an SCGB1A1-HRP conjugate. The assay sample and buffer are incubated together with SCGB1A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SCGB1A1 concentration since SCGB1A1 from samples and SCGB1A1-HRP conjugate compete for the anti-SCGB1A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SCGB1A1 from the sample, fewer sites are left to bind SCGB1A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SCGB1A1 concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Secretoglobin, Family 1A, Member 1/Clara Cell Specific Protein CC16 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between SCGB1A1 and analogues was observed.

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Summary of the Assay Procedure for Mouse Secretoglobin, Family 1A, Member 1/Clara Cell Specific Protein CC16 ELISA kit

Citations of Mouse Secretoglobin, Family 1A, Member 1/Clara Cell Specific Protein CC16 ELISA kit

E03U0019 has been referenced in the below publications：

8-Isoprostane is an early biomarker for oxidative stress in chlorine-induced acute lung injury.