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BlueGene Biotech Mouse Interleukin 12 ELISA kit

E03I0033 Mouse Interleukin 12 ELISA kit

The Mouse Interleukin 12 ELISA kit can be used to identify samples from the mouse species. Interleukin 12 can also be called  IL12, CLMF p35, CLMF1, CTL maturation factor (TcMF), Cytotoxic lymphocyte maturation factor 1, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL 12 subunit p35, IL12A, Interleukin 12 alpha chain, Interleukin 12 p35, Interleukin 12A, Natural killer cell stimulatory factor 1, CLMF, CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, IL12 subunit p35, IL12A, IL12A_HUMAN, Interleukin 12 p35, Interleukin 12A, Interleukin-12 subunit alpha, NFSK, NK cell stimulatory factor chain 1, NKSF1,  NK cell stimulatory factor chain 1.

Specifications of Mouse Interleukin 12 ELISA kit

Product Information

Cat. No.

E03I0033

Product Name

Mouse Interleukin 12 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000pg/ml

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/ml1 vial

STANDARD B (0.5mL)

50 pg/ml

1 vial

STANDARD C (0.5mL)

100 pg/ml

1 vial

STANDARD D (0.5mL)

250 pg/ml

1 vial

STANDARD E (0.5mL)

500 pg/ml

1 vial

STANDARD F (0.5mL)

1000 pg/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL 12 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 12 antibody and an IL 12-HRP conjugate. The assay sample and buffer are incubated together with IL 12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 12 concentration since IL 12 from samples and IL 12-HRP conjugate compete for the anti-IL 12 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 12 from the sample, fewer sites are left to bind IL 12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 12 concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Interleukin 12 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL 12 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Interleukin 12 ELISA kit

Summary of the Assay Procedure for Mouse Interleukin 12 ELISA kit

Citations of Mouse Interleukin 12 ELISA kit

E03I0033 has been referenced in the below publications:

Therapeutic Effect and Discussion of Immunological Mechanism of Compound Lidocaine Cream on Contact Der-matitis Model of Mice.

Development of rubella virus subunit vaccine and study on the identification of the key amino acids in antibody combining sites of E1 protein.

Pathological and immunological characterization of bluetongue virus serotype 1 infection in type I interferons blocked immunocompetent adult mice.

Therapeutic Effect and Discussion of Immunological Mechanism of Compound Lidocaine Cream on Contact Der-matitis Model of Mice.

Development of rubella virus subunit vaccine and study on the identification of the key amino acids in antibody combining sites of E1 protein.

Pathological and immunological characterization of bluetongue virus serotype 1 infection in type I interferons blocked immunocompetent adult mice.




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