En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Fatty Acid Synthase ELISA kit (E03F0043)

E03F0043 Mouse Fatty Acid Synthase ELISA kit

The Mouse Fatty Acid Synthase ELISA kit can be used to identify samples from the mouse species. Fatty Acid Synthase can also be called CD95 (phospho Y232), CD95 (phospho Tyr232), p-CD95 (Tyr232), FAS (phospho Y232), FAS (phospho Tyr232), p-FAS (Tyr232), ALPS 1A, ALPS1A, APO 1, Apo 1 antigen, APO 1 cell surface antigen, Apo-1 antigen, APO1, Apo1 antigen, APO1 cell surface antigen, Apoptosis antigen 1, Apoptosis mediating surface antigen FAS, Apoptosis-mediating surface antigen FAS, APT 1, APT1, CD 95, CD 95 antigen, Delta Fas, Delta Fas/APO 1/CD95, FAS 1, FAS 827dupA, Fas AMA, FAS, FAS Antigen, FAS1, FASLG receptor, FASTM, TNF receptor superfamily, member 6, TNFRSF 6, TNFRSF6, Tumor necrosis factor receptor superfamily member 6, FAS.

Products

Specifications of Mouse Fatty Acid Synthase ELISA kit

Product Information

Cat. No.

E03F0043

Product Name

Mouse Fatty Acid Synthase ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

5-100ug/ml

Sensitivity

1.0ug/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ug/ml

1 vial

STANDARD B (0.5mL)

5.0 ug/ml

1 vial

STANDARD C (0.5mL)

10 ug/ml

1 vial

STANDARD D (0.5mL)

25 ug/ml

1 vial

STANDARD E (0.5mL)

50 ug/ml

1 vial

STANDARD F (0.5mL)

100 ug/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

FAS ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FAS. Standards or samples are then added to the microtiter plate wells and FAS if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FAS present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FAS are added to each well to sandwich” the FAS immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FAS and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FAS concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Fatty Acid Synthase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between FAS selectin and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Fatty Acid Synthase ELISA kit

Summary of the Assay Procedure for Mouse Fatty Acid Synthase ELISA kit

Citations of Mouse Fatty Acid Synthase ELISA kit

E03F0043 has been referenced in the below publications:

Triglyceride with medium-chain fatty acids increases the activity and expression of hormone-sensitive lipase in white adipose tissue of C57BL/6J mice.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.