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  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Collagen Type IV ELISA kit (E03C0047)

E03C0047 Mouse Collagen Type IV ELISA kit

The Mouse Collagen Type IV ELISA kit can be used to identify samples from the mouse species. Collagen Type IV can also be called ColIV, Col4, or COL4.

Products

Specifications of Mouse Collagen Type IV ELISA kit

Product Information

Cat. No.

E03C0047

Product Name

Mouse Collagen Type IV ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000ng/mL

Sensitivity

1.0ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

COL4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-COL4 antibody and an COL4-HRP conjugate. The assay sample and buffer are incubated together with COL4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL4 concentration since COL4 from samples and COL4-HRP conjugate compete for the anti-COL4 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL4 from the sample, fewer sites are left to bind COL4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL4 concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Collagen Type IV ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between COL4 and analogues was observed.


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Summary of the Assay Procedure for Mouse Collagen Type IV ELISA kit

Summary of the Assay Procedure for Mouse Collagen Type IV ELISA kit

Citations of Mouse Collagen Type IV ELISA kit

E03C0047 has been referenced in the below publications:

The protective effect of 36 amino acid polypeptide fragment to CCl 4-induced liver fibrosis and its mechanism.


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