E03L0021 Mouse Luteinizing Hormone ELISA kit
The Mouse Luteinizing Hormone ELISA kit can be used to identify samples from the mouse species. Luteinizing Hormone can also be called ICSH, Lutropin, Interstitial Cell Stimulating Hormone, LH.
Specifications of Mouse Luteinizing Hormone ELISA kit
Product Information | |
Cat. No. | E03L0021 |
Product Name | Mouse Luteinizing Hormone ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD D (0.5mL) | 10 ng/ml | 1 vial |
STANDARD E (0.5mL) | 25 ng/ml | 1 vial |
STANDARD F (0.5mL) | 50 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
LH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-LH antibody and an LH-HRP conjugate. The assay sample and buffer are incubated together with LH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LH concentration since LH from samples and LH-HRP conjugate compete for the anti-LH antibody binding site. Since the number of sites is limited, as more sites are occupied by LH from the sample, fewer sites are left to bind LH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LH concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Luteinizing Hormone ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between LH and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Luteinizing Hormone ELISA kit
Citations of Mouse Luteinizing Hormone ELISA kit
E03L0021 has been referenced in the below publications：
Pulmonary exposure to cellulose nanocrystals caused deleterious effects to reproductive system in male mice.
The effect of the murine ovarian vitrification allograft for ovarian function in ovarian failure rat model.
