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  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Superoxide Dismutase ELISA kit (E03S0012)

E03S0012 Mouse Superoxide Dismutase ELISA kit

The Mouse Superoxide Dismutase ELISA kit can be used to identify samples from the mouse species. Superoxide Dismutase can also be called SOD1, Cu-Zn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble, ALS, ALS1, amyotrophic lateral sclerosis 1, adult, Cu, Zn superoxide dismutase, hSod1, indophenoloxidase A, IPOA, SOD, Superoxide dismutase 1, superoxide dismutase 1, soluble, superoxide dismutase, cystolic.

Products

Specifications of Mouse Superoxide Dismutase ELISA kit

Product Information

Cat. No.

E03S0012

Product Name

Mouse Superoxide Dismutase ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

0.5-10μg/ml

Sensitivity

0.1μg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

0.5 ug/mL

1 vial

STANDARD C (0.5mL)

1.0 ug/mL

1 vial

STANDARD D (0.5mL)

2.5 ug/mL

1 vial

STANDARD E (0.5mL)

5.0 ug/mL

1 vial

STANDARD F (0.5mL)

10 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SOD ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SOD antibody and an SOD-HRP conjugate. The assay sample and buffer are incubated together with SOD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SOD concentration since SOD from samples and SOD-HRP conjugate compete for the anti-SOD antibody binding site. Since the number of sites is limited, as more sites are occupied by SOD from the sample, fewer sites are left to bind SOD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SOD concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Superoxide Dismutase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SOD and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Superoxide Dismutase ELISA kit

Summary of the Assay Procedure for Mouse Superoxide Dismutase ELISA kit

Citations of Mouse Superoxide Dismutase ELISA kit

E03S0012 has been referenced in the below publications:

Lonicera caerulea Extract Attenuates Non-Alcoholic Fatty Liver Disease in Free Fatty Acid-Induced HepG2 Hepatocytes and in High Fat Diet-Fed Mice.

The research on antioxidant effect and active function of Tyr-Ala di-peptide.

Ameliorative effects of black ginseng on nonalcoholic fatty liver disease in free fatty acideinduced HepG2 cells and high-fat/highfructose diet-fed mice.

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