E03D0010 Mouse Diacyl Glycerol ELISA kit
The Mouse Diacyl Glycerol ELISA kit can be used to identify samples from the mouse species. Diacyl Glycerol can also be called DAG.
Specifications of Mouse Diacyl Glycerol ELISA kit
Product Information | |
Cat. No. | E03D0010 |
Product Name | Mouse Diacyl Glycerol ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD D (0.5mL) | 10 ng/ml | 1 vial |
STANDARD E (0.5mL) | 25 ng/ml | 1 vial |
STANDARD F (0.5mL) | 50 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
DAG ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-DAG antibody and an DAG-HRP conjugate. The assay sample and buffer are incubated together with DAG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DAG concentration since DAG from samples and DAG-HRP conjugate compete for the anti-DAG antibody binding site. Since the number of sites is limited, as more sites are occupied by DAG from the sample, fewer sites are left to bind DAG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DAG concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Diacyl Glycerol ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between DAG and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Diacyl Glycerol ELISA kit
Citations of Mouse Diacyl Glycerol ELISA kit
E03D0010 has been referenced in the below publications：
Effect of omega-3 fatty acids on glucose homeostasis: role of free fatty acid receptor 1.
Quercetin and lithium chloride potentiate the protective effects of carvedilol against renal ischemia-reperfusion injury in high-fructose, high-fat diet-fed Swiss albino mice independent of renal lipid signaling.
