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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Alanine Aminotransferase ELISA kit (E03A0388)

E03A0388 Mouse Alanine Aminotransferase ELISA kit

The Mouse Alanine Aminotransferase ELISA kit can be used to identify samples from the mouse species. Alanine Aminotransferase can also be called Alanine aminotransferase, AAT1, Alanine aminotransferase 1, ALT1, Glutamic alanine transaminase 1, Glutamic pyruvate transaminase, Glutamic pyruvic transaminase 1, GPT, GPT1, SGPT, ALAT1, ALT.

Products

Specifications of Mouse Alanine Aminotransferase ELISA kit

Product Information

Cat. No.

E03A0388

Product Name

Mouse Alanine Aminotransferase ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

10-250 U/L

Sensitivity

1.0U/L

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 U/L1 vial

STANDARD B (0.5mL)

10 U/L

1 vial

STANDARD C (0.5mL)

25 U/L

1 vial

STANDARD D (0.5mL)

50 U/L

1 vial

STANDARD E (0.5mL)

100 U/L

1 vial

STANDARD F (0.5mL)

250 U/L

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ALT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ALT antibody and an ALT-HRP conjugate. The assay sample and buffer are incubated together with ALT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALT concentration since ALT from samples and ALT-HRP conjugate compete for the anti-ALT antibody binding site. Since the number of sites is limited, as more sites are occupied by ALT from the sample, fewer sites are left to bind ALT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALT concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Alanine Aminotransferase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ALT and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Alanine Aminotransferase ELISA kit

Summary of the Assay Procedure for Mouse Alanine Aminotransferase ELISA kit

Citations of Mouse Alanine Aminotransferase ELISA kit

E03A0388 has been referenced in the below publications:

Fraxetin inhibits the growth of colon adenocarcinoma cells via the Janus kinase 2/signal transducer and activator of transcription 3 signalling pathway.


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