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BlueGene Biotech Mouse Purine Nucleoside Phosphorylase ELISA kit (E03P0618)

E03P0618 Mouse Purine Nucleoside Phosphorylase ELISA kit

The Mouse Purine Nucleoside Phosphorylase ELISA kit can be used to identify samples from the mouse species. Purine Nucleoside Phosphorylase can also be called NP, PUNP, Nucleoside Phosphorylase, Inosine phosphorylase, Inosine-guanosine phosphorylase, PNP.

Products

Specifications of Mouse Purine Nucleoside Phosphorylase ELISA kit

Product Information

Cat. No.

E03P0618

Product Name

Mouse Purine Nucleoside Phosphorylase ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

5.0-100ng/ml

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PNP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PNP antibody and an PNP-HRP conjugate. The assay sample and buffer are incubated together with PNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PNP concentration since PNP from samples and PNP-HRP conjugate compete for the anti-PNP antibody binding site. Since the number of sites is limited, as more sites are occupied by PNP from the sample, fewer sites are left to bind PNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PNP concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Purine Nucleoside Phosphorylase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PNP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Purine Nucleoside Phosphorylase ELISA kit

Summary of the Assay Procedure for Mouse Purine Nucleoside Phosphorylase ELISA kit

Citations of Mouse Purine Nucleoside Phosphorylase ELISA kit

E03P0618 has been referenced in the below publications:

HepatoprotectiVe effect of dioscin against APAP-induced acute liver damage in vitro and in vivo.

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