E03P0618 Mouse Purine Nucleoside Phosphorylase ELISA kit
The Mouse Purine Nucleoside Phosphorylase ELISA kit can be used to identify samples from the mouse species. Purine Nucleoside Phosphorylase can also be called NP, PUNP, Nucleoside Phosphorylase, Inosine phosphorylase, Inosine-guanosine phosphorylase, PNP.
Product Information | |
Cat. No. | E03P0618 |
Product Name | Mouse Purine Nucleoside Phosphorylase ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PNP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PNP antibody and an PNP-HRP conjugate. The assay sample and buffer are incubated together with PNP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PNP concentration since PNP from samples and PNP-HRP conjugate compete for the anti-PNP antibody binding site. Since the number of sites is limited, as more sites are occupied by PNP from the sample, fewer sites are left to bind PNP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PNP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PNP and analogues was observed. | |
E03P0618 has been referenced in the below publications：
HepatoprotectiVe effect of dioscin against APAP-induced acute liver damage in vitro and in vivo.
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