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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Glycated Hemoglobin A1c ELISA kit (E03G0322)

E03G0322 Mouse Glycated Hemoglobin A1c ELISA kit

The Mouse Glycated Hemoglobin A1c ELISA kit can be used to identify samples from the mouse species. Glycated Hemoglobin A1c can also be called Hb1c, HbAIc, Glycosylated Hemoglobin, Hemoglobin A1c, and GHbA1c.

Products

Specifications of Mouse Glycated Hemoglobin A1c ELISA kit

Product Information

Cat. No.

E03G0322

Product Name

Mouse Glycated Hemoglobin A1c ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

25-500ug/ml

Sensitivity

1.0ug/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/ml1 vial

STANDARD B (0.5mL)

25 ug/ml

1 vial

STANDARD C (0.5mL)

50 ug/ml

1 vial

STANDARD D (0.5mL)

100 ug/ml

1 vial

STANDARD E (0.5mL)

250 ug/ml

1 vial

STANDARD F (0.5mL)

500 ug/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GHbA1c ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GHbA1c antibody and an GHbA1c-HRP conjugate. The assay sample and buffer are incubated together with GHbA1c-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GHbA1c concentration since GHbA1c from samples and GHbA1c-HRP conjugate compete for the anti-GHbA1c antibody binding site. Since the number of sites is limited, as more sites are occupied by GHbA1c from the sample, fewer sites are left to bind GHbA1c-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GHbA1c concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Glycated Hemoglobin A1c ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GHbA1c and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Glycated Hemoglobin A1c ELISA kit

Summary of the Assay Procedure for Mouse Glycated Hemoglobin A1c ELISA kit

Citations of Mouse Glycated Hemoglobin A1c ELISA kit

E03G0322 has been referenced in the below publications:

Oxamate Improves Glycemic Control and Insulin Sensitivity via Inhibition of TissueLactate Production in db/db Mice.

The research on antioxidant effect and active function of Tyr-Ala di-peptide.

Oxamate Enhances the Anti-Inflammatory and Insulin-Sensitizing Effects of Metformin in Diabetic Mice.

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