E03G0322 Mouse Glycated Hemoglobin A1c ELISA kit
The Mouse Glycated Hemoglobin A1c ELISA kit can be used to identify samples from the mouse species. Glycated Hemoglobin A1c can also be called Hb1c, HbAIc, Glycosylated Hemoglobin, Hemoglobin A1c, and GHbA1c.
Product Information | |
Cat. No. | E03G0322 |
Product Name | Mouse Glycated Hemoglobin A1c ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500ug/ml |
Sensitivity | 1.0ug/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/ml | 1 vial |
STANDARD B (0.5mL) | 25 ug/ml | 1 vial |
STANDARD C (0.5mL) | 50 ug/ml | 1 vial |
STANDARD D (0.5mL) | 100 ug/ml | 1 vial |
STANDARD E (0.5mL) | 250 ug/ml | 1 vial |
STANDARD F (0.5mL) | 500 ug/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
GHbA1c ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GHbA1c antibody and an GHbA1c-HRP conjugate. The assay sample and buffer are incubated together with GHbA1c-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GHbA1c concentration since GHbA1c from samples and GHbA1c-HRP conjugate compete for the anti-GHbA1c antibody binding site. Since the number of sites is limited, as more sites are occupied by GHbA1c from the sample, fewer sites are left to bind GHbA1c-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GHbA1c concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between GHbA1c and analogues was observed. | |
E03G0322 has been referenced in the below publications：
Oxamate Improves Glycemic Control and Insulin Sensitivity via Inhibition of TissueLactate Production in db/db Mice.
The research on antioxidant effect and active function of Tyr-Ala di-peptide.
Oxamate Enhances the Anti-Inflammatory and Insulin-Sensitizing Effects of Metformin in Diabetic Mice.
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