E03O0321 Mouse Orexin A ELISA kit
The Mouse Orexin A ELISA kit can be used to identify samples from the mouse species. Orexin A can also be called Hypocretin-1, OX A.
Mouse Orexin A ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
OX A ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-OX A antibody and an OX A-HRP conjugate. The assay sample and buffer are incubated together with OX A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OX A concentration since OX A from samples and OX A-HRP conjugate compete for the anti-OX A antibody binding site. Since the number of sites is limited, as more sites are occupied by OX A from the sample, fewer sites are left to bind OX A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OX A concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between OX A and analogues was observed.
E03O0321 has been referenced in the below publications：
Increased orexin expression promotes sleep/wake disturbances in the SOD1-G93A mouse model of amyotrophic lateral sclerosis.
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