Mouse Prostaglandin E2 ELISA kit - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Mouse Prostaglandin E2 ELISA kit

E03P0012 Mouse Prostaglandin E2 ELISA kit

The Mouse Prostaglandin E2 ELISA kit can be used to identify samples from the mouse species. Prostaglandin E2 can also be called EP 2, EP2, Prostaglandin E Receptor EP2, PGE receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostaglandin E receptor 2 EP2 subtype, Prostaglandin E receptor 2 subtype EP2 53kDa, Prostaglandin E receptor 2 subtype EP2, Prostaglandin E2 receptor, Prostaglandin E2 receptor EP2 subtype, Prostanoid EP2 receptor, PTGER 2, PTGER2, PGE2.

Products

Specifications of Mouse Prostaglandin E2 ELISA kit

Product Information
Cat. No.	E03P0012
Product Name	Mouse Prostaglandin E2 ELISA kit
Species	Mouse
Product Size	48 Tests / 96 Tests
Concentration	2.5-50 ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	2.5 ng/mL	1 vial
STANDARD C (0.5mL)	5 ng/mL	1 vial
STANDARD D (0.5mL)	10 ng/mL	1 vial
STANDARD E (0.5mL)	25 ng/mL	1 vial
STANDARD F (0.5mL)	50 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

PGE2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PGE2 antibody and an PGE2-HRP conjugate. The assay sample and buffer are incubated together with PGE2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGE2 concentration since PGE2 from samples and PGE2-HRP conjugate compete for the anti-PGE2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PGE2 from the sample, fewer sites are left to bind PGE2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGE2 concentration in each sample is interpolated from this standard curve.

Quality Control on Mouse Prostaglandin E2 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between PGE2 and analogues was observed.