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  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Anti-Mullerian Hormone ELISA kit

E03A0433 Mouse Anti-Mullerian Hormone ELISA kit

The Mouse Anti-Mullerian Hormone ELISA kit can be used to identify samples from the mouse species. Anti-Mullerian Hormone can also be called AMH, MIS, antim, MIF, and anti-Mullerian hormone.

Specifications of Mouse Anti-Mullerian Hormone ELISA kit

Product Information

Cat. No.

E03A0433

Product Name

Mouse Anti-Mullerian Hormone ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

1.0-25ng/ml

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml1 vial

STANDARD B (0.5mL)

1.0 ng/ml

1 vial

STANDARD C (0.5mL)

2.5 ng/ml

1 vial

STANDARD D (0.5mL)

5.0 ng/ml

1 vial

STANDARD E (0.5mL)

10 ng/ml

1 vial

STANDARD F (0.5mL)

25 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AMH ELISA kit uses an anti-AMH antibody and an AMH-HRP conjugate in a competitive enzyme immunoassay method. AMH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-AMH antibody binding site between AMH from samples and AMH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of AMH. Since the number of sites is limited, as more sites are occupied by AMH from the sample, fewer sites are left to bind AMH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMH concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Anti-Mullerian Hormone ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AMH and analogues was observed.


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Summary of the Assay Procedure for Mouse Anti-Mullerian Hormone ELISA kit

Summary of the Assay Procedure for Mouse Anti-Mullerian Hormone ELISA kit

Citations of Mouse Anti-Mullerian Hormone ELISA kit

E03A0433 has been referenced in the below publications:

The effect of the murine ovarian vitrification allograft for ovarian function in ovarian failure rat model.

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