E03H1475 Mouse Interleukin 4, High Sensitivity ELISA kit
The Mouse Interleukin 4, High Sensitivity ELISA kit can be used to identify samples from the mouse species. Interleukin 4, High Sensitivity can also be called HS IL-4.
Specifications of Mouse Interleukin 4, High Sensitivity ELISA kit
Product Information | |
Cat. No. | E03H1475 |
Product Name | Mouse Interleukin 4, High Sensitivity ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 10 pg/mL | 1 vial |
STANDARD C (0.5mL) | 25 pg/mL | 1 vial |
STANDARD D (0.5mL) | 50 pg/mL | 1 vial |
STANDARD E (0.5mL) | 100 pg/mL | 1 vial |
STANDARD F (0.5mL) | 250 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HS IL-4 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-HS IL-4 antibody and an HS IL-4-HRP conjugate. The assay sample and buffer are incubated together with HS IL-4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HS IL-4 concentration since HS IL-4 from samples and HS IL-4-HRP conjugate compete for the anti-HS IL-4 antibody binding site. Since the number of sites is limited, as more sites are occupied by HS IL-4 from the sample, fewer sites are left to bind HS IL-4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HS IL-4 concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Interleukin 4, High Sensitivity ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HS IL-4 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Interleukin 4, High Sensitivity ELISA kit
Citations of Mouse Interleukin 4, High Sensitivity ELISA kit
E03H1475 has been referenced in the below publications：
Downregulation of Orai1 expression in the airway alleviates murine allergic rhinitis.
