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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Epinephrine/Adrenaline ELISA kit

E07A0007 Porcine Epinephrine/Adrenaline ELISA kit

Porcine Epinephrine/Adrenaline ELISA kit is suitable for detecting samples from porcine species. Epinephrine/Adrenaline can also be called ADR, EPI.

Specifications of Porcine Epinephrine/Adrenaline ELISA kit

Product Information

Cat. No.

E07A0007

Product Name

Porcine Epinephrine/Adrenaline ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

5-100 ng/mL

Sensitivity

1.0 ng/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

EPI ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EPI antibody and an EPI-HRP conjugate. The assay sample and buffer are incubated together with EPI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EPI concentration since EPI from samples and EPI-HRP conjugate compete for the anti-EPI antibody binding site. Since the number of sites is limited, as more sites are occupied by EPI from the sample, fewer sites are left to bind EPI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EPI concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Epinephrine/Adrenaline ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

94-105%

Linearity

Diluent Ratio

Range %

1:2

91-105

1:4

88-109

1:8

96-102

Specificity/Cross-reactivity

No significant cross-reactivity or interference between EPI and analogues was observed.



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Summary of the Assay Procedure for Porcine Epinephrine/Adrenaline ELISA kit

Summary of the Assay Procedure for Porcine Epinephrine/Adrenaline ELISA kit

Citations of Porcine Epinephrine/Adrenaline ELISA kit

E07A0007 has been referenced in the below publications:

Correlation between three glycometabolic-related hormones and muscle glycolysis, as well as meat quality, in three pig breeds.

Meat and nutritional quality comparison of purebred and crossbred pigs.

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