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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Thymic Stromal Lymphopoietin ELISA kit (E07T0353)

E07T0353 Porcine Thymic Stromal Lymphopoietin ELISA kit

The Porcine Thymic Stromal Lymphopoietin ELISA kit can be used to identify samples from the Porcine species. Thymic Stromal Lymphopoietin can also be called Thymic stromal lymphopoietin, Thymic stromal lymphopoietin isoform 1, Thymic stromal lymphopoietin protein TSLP, TSLP protein.

Products

Specifications of Porcine Thymic Stromal Lymphopoietin ELISA kit

Product Information

Cat. No.

E07T0353

Product Name

Porcine Thymic Stromal Lymphopoietin ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

100-2500pg/mL

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TSLP ELISA kit uses an anti-TSLP antibody and an TSLP-HRP conjugate in a competitive enzyme immunoassay method. TSLP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TSLP antibody binding site between TSLP from samples and TSLP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TSLP. Since the number of sites is limited, as more sites are occupied by TSLP from the sample, fewer sites are left to bind TSLP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TSLP concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Thymic Stromal Lymphopoietin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TSLP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Porcine Thymic Stromal Lymphopoietin ELISA kit

Summary of the Assay Procedure for Porcine Thymic Stromal Lymphopoietin ELISA kit

Citations of Porcine Thymic Stromal Lymphopoietin ELISA kit

E07T0353 has been referenced in the below publications:

Inflammatory Responses of Porcine MoDC and Intestinal Epithelial Cells in a Direct-Contact Co-culture System Following a Bacterial Challenge.

The Inflammatory Response to Enterotoxigenic E. coli and Probiotic E. faecium in a Coculture Model of Porcine Intestinal Epithelial and Dendritic Cells.

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