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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Nuclear factor Kb p65 ELISA kit

E07N0021 Porcine Nuclear factor Kb p65 ELISA kit

The Porcine Nuclear factor Kb p65 ELISA kit can be used to identify samples from the porcine species. Nuclear factor Kb p65 can also be called NFkB p65, NF kB P65, NFKBp65, p65 NF kappaB, p65 NFkB, RELA, Transcription Factor p65.

Specifications of Porcine Nuclear factor Kb p65 ELISA kit

Product Information

Cat. No.

E07N0021

Product Name

Porcine Nuclear factor Kb p65 ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

5-100 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NFkB p65 ELISA kit uses an anti-NFkB p65 antibody and an NFkB p65-HRP conjugate in a competitive enzyme immunoassay method. NFkB p65-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NFkB p65 antibody binding site between NFkB p65 from samples and NFkB p65-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NFkB p65. Since the number of sites is limited, as more sites are occupied by NFkB p65 from the sample, fewer sites are left to bind NFkB p65-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NFkB p65 concentration in each sample is interpolated from tNFkB p65 standard curve.


Quality Control on Porcine Nuclear factor Kb p65 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NFkB p65 and analogues was observed.


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Summary of the Assay Procedure for Porcine Nuclear factor Kb p65 ELISA kit

Summary of the Assay Procedure for Porcine Nuclear factor Kb p65 ELISA kit

Citations of Porcine Nuclear factor Kb p65 ELISA kit

E07N0021 has been referenced in the below publications:

The effects of baicalin on NF-kB and NLRP3 signaling pathway in piglets peripheral blood mononuclear cells subjected to immunological stress.

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