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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Glucose, Free ELISA kit

E07G0030 Porcine Glucose,Free ELISA kit

The Porcine Glucose,Free ELISA kit can be used to identify samples from the porcine species. Glucose,Free can also be called GBA, GC, GLUC, GBA1, Beta-GC, Acid beta-glucosidase, Alglucerase, Beta-glucocerebrosidase, Beta-glucosylceramidase 1.

Specifications of Porcine Glucose, Free ELISA kit

Product Information

Cat. No.

E07G0030

Product Name

Porcine Glucose, Free ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GF ELISA kit uses an anti-GF antibody and an GF-HRP conjugate in a competitive enzyme immunoassay method. GF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GF antibody binding site between GF from samples and GF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GF. Since the number of sites is limited, as more sites are occupied by GF from the sample, fewer sites are left to bind GF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GF concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Glucose, Free ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GF and analogues was observed.


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Summary of the Assay Procedure for Porcine Glucose, Free ELISA kit

Summary of the Assay Procedure for Porcine Glucose, Free ELISA kit

Citations of Porcine Glucose, Free ELISA kit

E07G0030 has been referenced in the below publications:

Comprehensive Analysis of lncRNAs and circRNAs Reveals the Metabolic Specialization in Oxidative and Glycolytic Skeletal Muscles.

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