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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine High Mobility Group box1 Protein ELISA kit

  • Epigenetics and Nuclear Signaling

E07H0009 Porcine High Mobility Group box1 Protein ELISA kit

The Porcine High Mobility Group box1 Protein ELISA kit can be used to identify samples from the porcine species. High Mobility Group box1 Protein can also be called HMGB1, HMG1, HMG3, SBP1, Sulfoglucuronyl Carbohydrate Binding Protein, Amphoterin, High Mobility Group Box 1 Protein.

Specifications of Porcine High Mobility Group box1 Protein ELISA kit

Product Information

Cat. No.

E07H0009

Product Name

Porcine High Mobility Group box1 Protein ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

5-100 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HMGB1 ELISA kit uses an anti-HMGB1 antibody and an HMGB1-HRP conjugate in a competitive enzyme immunoassay method. HMGB1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HMGB1 antibody binding site between HMGB1 from samples and HMGB1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HMGB1. Since the number of sites is limited, as more sites are occupied by HMGB1 from the sample, fewer sites are left to bind HMGB1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMGB1 concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine High Mobility Group box1 Protein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HMGB1 and analogues was observed.


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Summary of the Assay Procedure for Porcine High Mobility Group box1 Protein ELISA kit

Summary of the Assay Procedure for Porcine High Mobility Group box1 Protein ELISA kit

Citations of Porcine High Mobility Group box1 Protein ELISA kit

E07H0009 has been referenced in the below publications:

Effects of direct hemoperfusion with polyvinylidene difluoride-serine column in experimental sepsis.

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