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E07C0086 Porcine Catalase ELISA Kit

Porcine Catalase ELISA kit is suitable for the detection of samples from porcine species. Catalase can also be called as Cas1; CAT; Cs1.

Specifications Of E07C0086 Porcine CAT ELISA Kit

Porduct's Information

Cat.NO.

E07C0086

Porduct's Name

Porcine Catalase ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

1-25 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial



Principle of the Assay

CAT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CAT antibody and an CAT-HRP conjugate. The assay sample and buffer are incubated together with CAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAT concentration since CAT from samples and CAT-HRP conjugate compete for the anti-CAT antibody binding site. Since the number of sites is limited, as more sites are occupied by CAT from the sample, fewer sites are left to bind CAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAT concentration in each sample is interpolated from this standard curve.


Quality Control On Porcine CAT ELISA Kit

CV

Intra Variation% <10%

Inter Variation% <12%

Recovery%

95-106

Linearity

Diluent ratio

Range %

1:2

90-102

1:4

86-114

1:8

94-105

Specificity/

Cross-reactivity

No significant cross-reactivity or interference between CAT and analogues was observed.


BlueGene Biotech Product Show

Summary Of The Assay Procedures For E07C0086 Porcine CAT ELISA Kit

Summary Of The Assay Procedures For E07C0086 Porcine CAT ELISA Kit

Citations Of Porcine Catalase ELISA Kit

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