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BlueGene Biotech Porcine Lipopolysaccharides/lipooligosaccharide ELISA kit (E07L0268)

E07L0268 Porcine Lipopolysaccharides/lipooligosaccharide ELISA kit

The Porcine Lipopolysaccharides/lipooligosaccharide ELISA kitcan be used to identify samples from the porcine species. Lipopolysaccharides/lipooligosaccharide can also be called Lipoglycans,LPS, Lipooligosaccharide, Lipo-Oligosaccharide, Endotoxin.

Products

Specifications of Porcine Lipopolysaccharides/lipooligosaccharide ELISA kit

Product Information

Cat. No.

E07L0268

Product Name

Porcine Lipopolysaccharides/lipooligosaccharide ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

25-500 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

LPS/LOS ELISA kit uses an anti-LPS/LOS antibody and an LPS/LOS-HRP conjugate in a competitive enzyme immunoassay method. LPS/LOS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-LPS/LOS antibody binding site between LPS/LOS from samples and LPS/LOS-HRP conjugate, the intensity of the color is inversely proportional to the concentration of LPS/LOS. Since the number of sites is limited, as more sites are occupied by LPS/LOS from the sample, fewer sites are left to bind LPS/LOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LPS/LOS concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Lipopolysaccharides/lipooligosaccharide ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between LPS/LOS and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Porcine Lipopolysaccharides/lipooligosaccharide ELISA kit

Summary of the Assay Procedure for Porcine Lipopolysaccharides/lipooligosaccharide ELISA kit

Citations of Porcine Lipopolysaccharides/lipooligosaccharide ELISA kit

E07L0268 has been referenced in the below publications:

Changes in cecal morphology, cell proliferation, antioxidant enzyme, volatile fatty acids, lipopolysaccharide, and cytokines in piglets during the postweaning period.

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