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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Estrogens ELISA kit

E07E0314 Porcine Estrogens ELISA kit

The Porcine Estrogens ELISA kit is suitable for the detection of samples from porcine species. Estrogens can also be called ES, oestrogen.


Specifications of Porcine Estrogens ELISA kit

Product Information

Cat. No.

E07E0314

Product Name

Porcine Estrogens ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ES ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ES antibody and an ES-HRP conjugate. The assay sample and buffer are incubated together with ES-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ES concentration since ES from samples and ES-HRP conjugate compete for the anti-ES antibody binding site. Since the number of sites is limited, as more sites are occupied by ES from the sample, fewer sites are left to bind ES-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ES concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Estrogens ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-110%

Linearity

Diluent Ratio

Range %

1:2

93-110

1:4

89-107

1:8

91-105

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ES and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Porcine Estrogens ELISA kit

Summary of the Assay Procedure for Porcine Estrogens ELISA kit

Citations of Porcine Estrogens ELISA kit

E07E0314 has been referenced in the below publications:

Expression of CART Protein in Pig Ovarian Follicles and Effects on Folliclar Granulosa Cells in Vitro Culture.

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