Porcine Total Adiponectin ELISA kit is suitable for the detection of samples from porcine species. Total Adiponectin can also be called as 30 kDa adipocyte complement related protein; 30 kDa adipocyte complement-related protein; ACDC; ACRP 30; ACRP30; ADIPO; Adipocyte; Adipocyte C1q and collagen domain containing protein; Adipocyte complement related 30 kDa protein; Adipocyte complement related protein of 30 kDa; Adipocyte complement-related 30 kDa protein; Adiponectin; AdipoQ; Adipose most abundant gene transcript 1; Adipose most abundant gene transcript 1 protein; Adipose specific collagen like factor; ADIPQTL1; ADPN; APM 1; apM-1; ApM1; C1q and collagen domain-containing protein; GBP 28; GBP28; Gelatin binding protein; Gelatin binding protein 28; Gelatin-binding protein.
Specifications Of E07A0125 Porcine ADPN ELISA Kit
Porduct's Information | |
Cat.NO. | E07A0125 |
Porduct's Name | Porcine Total Adiponectin ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 1-25 ug/mL |
Sensitivity | 0.1 ug/mL |
Principal | sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ug/mL | 1 vial |
STANDARD B (0.5mL) | 1 ug/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ug/mL | 1 vial |
STANDARD D (0.5mL) | 5 ug/mL | 1 vial |
STANDARD E (0.5mL) | 10 ug/mL | 1 vial |
STANDARD F (0.5mL) | 25 ug/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ADPN ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ADPN antibody and an ADPN-HRP conjugate. The assay sample and buffer are incubated together with ADPN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADPN concentration since ADPN from samples and ADPN-HRP conjugate compete for the anti-ADPN antibody binding site. Since the number of sites is limited, as more sites are occupied by ADPN from the sample, fewer sites are left to bind ADPN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADPN concentration in each sample is interpolated from this standard curve. |
Quality Control On Porcine ADPN ELISA Kit
CV | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery% | 91-105 | |
Linearity | Diluent ratio | Range % |
1:2 | 95-104 | |
1:4 | 89-110 | |
1:8 | 96-107 | |
Specificity/ Cross-reactivity | No significant cross-reactivity or interference between ADPN and analogues was observed. | |
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Summary Of The Assay Procedures For E07A0125 Porcine ADPN ELISA Kit
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