Porcine Ghrelin ELISA kit is suitable for the detection of samples from porcine species. Ghrelin can also be called as Appetite regulating hormone; Ghrelin 27; Ghrelin 28; Ghrelin/obestatin prepropeptide; Growth hormone releasing peptide; Growth hormone secretagogue; M46 protein; Motilin related peptide; MTLRP; Obestatin; Obestatin preprohormone.
Specifications Of E07G0013 Porcine Ghrelin ELISA Kit
Porduct's Information | |
Cat.NO. | E07G0013 |
Porduct's Name | Porcine Ghrelin ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Ghrelin ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Ghrelin antibody and an Ghrelin-HRP conjugate. The assay sample and buffer are incubated together with Ghrelin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Ghrelin concentration since Ghrelin from samples and Ghrelin-HRP conjugate compete for the anti-Ghrelin antibody binding site. Since the number of sites is limited, as more sites are occupied by Ghrelin from the sample, fewer sites are left to bind Ghrelin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Ghrelin concentration in each sample is interpolated from this standard curve. |
Quality Control On Porcine Ghrelin ELISA Kit
CV | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery% | 94-108 | |
Linearity | Diluent ratio | Range % |
1:2 | 97-114 | |
1:4 | 93-105 | |
1:8 | 91-106 | |
Specificity/ Cross-reactivity | No significant cross-reactivity or interference between Ghrelin and analogues was observed. | |
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Summary Of The Assay Procedures For E07G0013 Porcine Ghrelin ELISA Kit
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