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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Ghrelin ELISA kit

E07G0013 Porcine Ghrelin ELISA kit

The Porcine Ghrelin ELISA kit is suitable for the detection of samples from porcine species. Ghrelin can also be called Appetite regulating hormone, Ghrelin 27, Ghrelin 28, Ghrelin/obestatin prepropeptide, Growth hormone releasing peptide, Growth hormone secretagogue, M46 protein, Motilin related peptide, MTLRP, Obestatin, Obestatin preprohormone.

Specifications of Porcine Ghrelin ELISA kit

Product Information

Cat. No.

E07G0013

Product Name

Porcine Ghrelin ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

5-100 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Ghrelin ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Ghrelin antibody and an Ghrelin-HRP conjugate. The assay sample and buffer are incubated together with Ghrelin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Ghrelin concentration since Ghrelin from samples and Ghrelin-HRP conjugate compete for the anti-Ghrelin antibody binding site. Since the number of sites is limited, as more sites are occupied by Ghrelin from the sample, fewer sites are left to bind Ghrelin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Ghrelin concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Ghrelin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

94-108%

Linearity

Diluent Ratio

Range %

1:2

97-114

1:4

93-105

1:8

91-106

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Ghrelin and analogues was observed.


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Summary of the Assay Procedure for Porcine Ghrelin ELISA kit

Summary of the Assay Procedure for Porcine Ghrelin ELISA kit

Citations of Porcine Ghrelin ELISA kit

E07G0013 has been referenced in the below publications:

The heat treatment and the gelation are strong determinants of the kinetics of milk proteins digestion and of the peripheral availability of amino acids.

Acid and rennet gels exhibit strong differences in the kinetics of milk protein digestion and amino acid bioavailability.

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