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E07D0214 Porcine Deoxyribonuclease I ELISA Kit

  • Epigenetics and Nuclear Signaling

Porcine Deoxyribonuclease I ELISA kit is suitable for the detection of samples from porcine species. Deoxyribonuclease I can also be called as DNaseI; DNL1; DRNI; DNase-I; Dornase alfa.

Specifications Of E07D0214 Porcine Dnase I ELISA Kit

Porduct's Information

Cat.NO.

E07D0214

Porduct's Name

Porcine Deoxyribonuclease I ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Dnase I ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Dnase I antibody and an Dnase I-HRP conjugate. The assay sample and buffer are incubated together with Dnase I-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Dnase I concentration since Dnase I from samples and Dnase I-HRP conjugate compete for the anti-Dnase I antibody binding site. Since the number of sites is limited, as more sites are occupied by Dnase I from the sample, fewer sites are left to bind Dnase I-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Dnase I concentration in each sample is interpolated from this standard curve.


Quality Control On Porcine Dnase I ELISA Kit

CV

Intra Variation% <10%

Inter Variation% <12%

Recovery%

94-116

Linearity

Diluent ratio

Range %

1:2

98-110

1:4

96-107

1:8

93-108

Specificity/

Cross-reactivity

No significant cross-reactivity or interference between Dnase I and analogues was observed.


BlueGene Biotech Product Show

Summary Of The Assay Procedures For E07D0214 Porcine Dnase I ELISA Kit

Summary Of The Assay Procedures For E07D0214 Porcine Dnase I ELISA Kit

Citations Of Porcine Deoxyribonuclease I ELISA Kit

Fresh-frozen plasma resuscitation after traumatic brain injury and shock attenuates extracellular nucleosome levels and deoxyribonuclease 1 depletion

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