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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Cortisol ELISA kit (E07C0008)

E07C0008 Porcine Cortisol ELISA kit

The Porcine Cortisol ELISA kit is suitable for detecting samples from porcine species. Cortisol can also be called CORT, Corticosrone.

Products

Specifications of Porcine Cortisol ELISA kit

Product Information

Cat. No.

E07C0008

Product Name

Porcine Cortisol ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CORT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CORT antibody and an CORT-HRP conjugate. The assay sample and buffer are incubated together with CORT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CORT concentration since CORT from samples and CORT-HRP conjugate compete for the anti-CORT antibody binding site. Since the number of sites is limited, as more sites are occupied by CORT from the sample, fewer sites are left to bind CORT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CORT concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Cortisol ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

92-106%

Linearity

Diluent Ratio

Range %

1:2

95-114

1:4

92-108

1:8

94-105

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CORT and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Porcine Cortisol ELISA kit

Summary of the Assay Procedure for Porcine Cortisol ELISA kit

Citations of Porcine Cortisol ELISA kit

E07C0008 has been referenced in the below publications:

The Experimental and Clinical Study of Laparoendoscopic Single-site Surgery.

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