Name: Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit
Brand: Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit

E07C0209 Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit

Porcine soluble Cluster of differentiation 40 ligand ELISA kit is suitable for the detection of samples from porcine species. soluble Cluster of differentiation 40 ligand can also be called CD40LG, CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L, CD40 ligand, Scd40l.

Products

Specifications of Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit

Product Information
Cat. No.	E07C0209
Product Name	Porcine soluble Cluster of differentiation 40 ligand ELISA kit
Species	Porcine
Product Size	48 Tests / 96 Tests
Concentration	2.5-50 ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	2.5 ng/mL	1 vial
STANDARD C (0.5mL)	5 ng/mL	1 vial
STANDARD D (0.5mL)	10 ng/mL	1 vial
STANDARD E (0.5mL)	25 ng/mL	1 vial
STANDARD F (0.5mL)	50 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

Scd40l ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Scd40l antibody and an Scd40l-HRP conjugate. The assay sample and buffer are incubated together with Scd40l-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Scd40l concentration since Scd40l from samples and Scd40l-HRP conjugate compete for the anti-Scd40l antibody binding site. Since the number of sites is limited, as more sites are occupied by Scd40l from the sample, fewer sites are left to bind Scd40l-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Scd40l concentration in each sample is interpolated from this standard curve.

Quality Control on Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	93-108%
Linearity	Diluent Ratio	Range %
	1:2	95-112
	1:4	91-106
	1:8	93-103
Specificity/Cross-reactivity	No significant cross-reactivity or interference between Scd40l and analogues was observed.

BlueGene Biotech Product Show

Summary of the Assay Procedure for Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit

Citations of Porcine Soluble Cluster Of Differentiation 40 Ligand ELISA kit

E07C0209 has been referenced in the below publications:

Treatment with a histone deacetylase inhibitor, valproic acid, is associated with increased platelet activation in a large animal model of traumatic brain injury and hemorrhagic shock.