E07T0871 Porcine Trefoil Factor ELISA kit
The Porcine Trefoil Factor ELISA kit can be used to identify samples from the porcine species. Trefoil Factor can also be called TFF.
Specifications of Porcine Trefoil Factor ELISA kit
Product Information | |
Cat. No. | E07T0871 |
Product Name | Porcine Trefoil Factor ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TFF ELISA kit uses an anti-TFF antibody and an TFF-HRP conjugate in a competitive enzyme immunoassay method. TFF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TFF antibody binding site between TFF from samples and TFF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TFF. Since the number of sites is limited, as more sites are occupied by TFF from the sample, fewer sites are left to bind TFF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TFF concentration in each sample is interpolated from this standard curve. |
Quality Control on Porcine Trefoil Factor ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TFF and analogues was observed. | |
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Summary of the Assay Procedure for Porcine Trefoil Factor ELISA kit
Citations of Porcine Trefoil Factor ELISA kit
E07T0871 has been referenced in the below publications：
Dietary fibre affects intestinal mucosal barrier function and regulates intestinal bacteria in weaning piglets.
