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E07D0277 Porcine Diamine ELISA Kit

Porcine Diamine dopamine ELISA kit is suitable for the detection of samples from porcine species. Diamine can also be called as 2-(3,4-Dihydroxyphenyl)ethylamine; 3,4-Dihydroxyphenethylamine; 3-Hydroxytyramine; Intropin; Revivan; Oxytyramine; Dopamine Hydrochloride.

Specifications Of E07D0277 Porcine DA ELISA Kit

Porduct's Information

Cat.NO.

E07D0277

Porduct's Name

Porcine Diamine ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

10-250 ng/dl

Sensitivity

1.0 ng/dl

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFIDAION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/dl

1 vial

STANDARD B (0.5mL)

10 ng/dl

1 vial

STANDARD C (0.5mL)

25 ng/dl

1 vial

STANDARD D (0.5mL)

50 ng/dl

1 vial

STANDARD E (0.5mL)

100 ng/dl

1 vial

STANDARD F (0.5mL)

250 ng/dl

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

DA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-DA antibody and an DA-HRP conjugate. The assay sample and buffer are incubated together with DA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DA concentration since DA from samples and DA-HRP conjugate compete for the anti-DA antibody binding site. Since the number of sites is limited, as more sites are occupied by DA from the sample, fewer sites are left to bind DA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DA concentration in each sample is interpolated from this standard curve.


Quality Control On Porcine DA ELISA Kit

CV

Intra Variation% <10%

Inter Variation% <12%

Recovery%

91-106

Linearity

Diluent ratio

Range %

1:2

95-102

1:4

88-107

1:8

90-105

Specificity/

Cross-reactivity

No significant cross-reactivity or interference between DA and analogues was observed.


BlueGene Biotech Product Show

Summary Of The Assay Procedures For E07D0277 Porcine DA ELISA Kit

Summary Of The Assay Procedures For E07D0277 Porcine DA ELISA Kit

Citations Of Porcine Diamine ELISA Kit

Positive effects of a Clostridium butyricum-based compound probiotic on growth performance, immune responses, intestinal morphology, hypothalamic neurotransmitters, and colonic microbiota in weaned piglets

Product: E07H0106 Porcine Serotonin/5 Hydroxytryptamine ELISA kit; E07G0074 Porcine Gamma Aminobutyric Acid ELISA kit;

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