Name: Porcine dopamine ELISA Kit
Brand: Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Porcine dopamine ELISA Kit

E07D0277 Porcine dopamine ELISA Kit

The Porcine dopamine ELISA Kit is suitable for detecting samples from porcine species. dopamine can also be called DA, 2-(3,4-Dihydroxyphenyl)ethylamine, 3,4-Dihydroxyphenethylamine, 3-Hydroxytyramine, Intropin, Revivan, Oxytyramine, Dopamine Hydrochloride.

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Specifications of Porcine dopamine ELISA Kit

Product Information
Cat. No.	E07D0277
Product Name	Porcine dopamine ELISA Kit
Species	Porcine
Product Size	48 Tests / 96 Tests
Concentration	10-250 ng/dl
Sensitivity	1.0 ng/dl
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFIDAION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/dl	1 vial
STANDARD B (0.5mL)	10 ng/dl	1 vial
STANDARD C (0.5mL)	25 ng/dl	1 vial
STANDARD D (0.5mL)	50 ng/dl	1 vial
STANDARD E (0.5mL)	100 ng/dl	1 vial
STANDARD F (0.5mL)	250 ng/dl	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

DA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-DA antibody and an DA-HRP conjugate. The assay sample and buffer are incubated together with DA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DA concentration since DA from samples and DA-HRP conjugate compete for the anti-DA antibody binding site. Since the number of sites is limited, as more sites are occupied by DA from the sample, fewer sites are left to bind DA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DA concentration in each sample is interpolated from this standard curve.

Quality Control on Porcine dopamine ELISA Kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	91-106%
Linearity	Diluent Ratio	Range %
	1:2	95-102
	1:4	88-107
	1:8	90-105
Specificity/Cross-reactivity	No significant cross-reactivity or interference between DA and analogues was observed.

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Summary of the Assay Procedure for Porcine dopamine ELISA Kit

Citations of Porcine dopamine ELISA Kit

E07D0277 has been referenced in the below publications:

Positive effects of a Clostridium butyricum-based compound probiotic on growth performance, immune responses, intestinal morphology, hypothalamic neurotransmitters, and colonic microbiota in weaned piglets.