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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Mycobacterium Tuberculosis Antibody ELISA kit (E07M0338)

E07M0338 Porcine Mycobacterium Tuberculosis Antibody ELISA kit

The Porcine Mycobacterium Tuberculosis Antibody ELISA kit can be used to identify samples from the porcine species. Mycobacterium Tuberculosis Antibody can also be called MTB-Ab.

Products

Specifications of Porcine Mycobacterium Tuberculosis Antibody ELISA kit

Product Information

Cat. No.

E07M0338

Product Name

Porcine Mycobacterium Tuberculosis Antibody ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ug/mL

Sensitivity

0.1 ug/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

0.5 ug/mL

1 vial

STANDARD C (0.5mL)

1.0 ug/mL

1 vial

STANDARD D (0.5mL)

2.5 ug/mL

1 vial

STANDARD E (0.5mL)

5.0 ug/mL

1 vial

STANDARD F (0.5mL)

10 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MTB Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing a MTB antigen and an MTB Ab-HRP conjugate. The assay sample and buffer are incubated together with MTB Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MTB Ab concentration since MTB Ab from samples and MTB Ab-HRP conjugate compete for the MTB antigen binding site. Since the number of sites is limited, as more sites are occupied by MTB Ab from the sample, fewer sites are left to bind MTB Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MTB Ab concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Mycobacterium Tuberculosis Antibody ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MTB Ab and analogues was observed.


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Summary of the Assay Procedure for Porcine Mycobacterium Tuberculosis Antibody ELISA kit

Summary of the Assay Procedure for Porcine Mycobacterium Tuberculosis Antibody ELISA kit

Citations of Porcine Mycobacterium Tuberculosis Antibody ELISA kit

E07M0338 has been referenced in the below publications:

Major Infectious Diseases Susceptible to sFat-1 Transgenic Pigs.

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