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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Histamine ELISA kit

E07H0013 Porcine Histamine ELISA kit

The Porcine Histamine ELISA kit can be used to identify samples from the porcine species. Histamine can also be called HIS, Histamine, Hist.

Specifications of Porcine Histamine ELISA kit

Product Information

Cat. No.

E07H0013

Product Name

Porcine Histamine ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

5-100 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HIS ELISA kit uses an anti-HIS antibody and an HIS-HRP conjugate in a competitive enzyme immunoassay method. HIS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HIS antibody binding site between HIS from samples and HIS-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HIS. Since the number of sites is limited, as more sites are occupied by HIS from the sample, fewer sites are left to bind HIS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HIS concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Histamine ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HIS and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Porcine Histamine ELISA kit

Summary of the Assay Procedure for Porcine Histamine ELISA kit

Citations of Porcine Histamine ELISA kit

E07H0013 has been referenced in the below publications:

Porcine mast cells infected with H1N1 influenza virus release histamine and inflammatory cytokines and chemokines.

Histamine contributes to severe pneumonia in pigs infected with 2009 pandemic H1N1 influenza virus.


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