E07I0004 Porcine Insulin ELISA kit
The Porcine Insulin ELISA kit can be used to identify samples from the porcine species. Insulin can also be called ILPR,Insulin A chain, Insulin B chain,IRDN.
Specifications of Porcine Insulin ELISA kit
Product Information | |
Cat. No. | E07I0004 |
Product Name | Porcine Insulin ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/ml |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
INS ELISA kit uses an anti-INS antibody and an INS-HRP conjugate in a competitive enzyme immunoassay method. INS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-INS antibody binding site between INS from samples and INS-HRP conjugate, the intensity of the color is inversely proportional to the concentration of INS. Since the number of sites is limited, as more sites are occupied by INS from the sample, fewer sites are left to bind INS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The INS concentration in each sample is interpolated from this standard curve. |
Quality Control on Porcine Insulin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between INS and analogues was observed. | |
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Summary of the Assay Procedure for Porcine Insulin ELISA kit
Citations of Porcine Insulin ELISA kit
E07I0004 has been referenced in the below publications：
Correlation between three glycometabolic-related hormones and muscle glycolysis, as well as meat quality, in three pig breeds.
Meat and nutritional quality comparison of purebred and crossbred pigs.
