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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Alpha-galactosyl ELISA kit

E07A0482 Porcine Alpha-galactosyl ELISA kit

The Porcine Alpha-galactosyl ELISA kit is suitable for the detection of samples from porcine species. Alpha-galactosyl can also be called Alpha-Gal, a-galactosyl, a-Gal, aGal.

Specifications of Porcine Alpha-galactosyl ELISA kit

Product Information

Cat. No.

E07A0482

Product Name

Porcine Alpha-galactosyl ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

1-25 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

a-Gal ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-a-Gal antibody and an a-Gal-HRP conjugate. The assay sample and buffer are incubated together with a-Gal-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the a-Gal concentration since a-Gal from samples and a-Gal-HRP conjugate compete for the anti-a-Gal antibody binding site. Since the number of sites is limited, as more sites are occupied by a-Gal from the sample, fewer sites are left to bind a-Gal-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The a-Gal concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Alpha-galactosyl ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

97-108%

Linearity

Diluent Ratio

Range %

1:2

91-105

1:4

88-111

1:8

93-107

Specificity/Cross-reactivity

No significant cross-reactivity or interference between a-Gal and analogues was observed.



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Summary of the Assay Procedure for Porcine Alpha-galactosyl ELISA kit

Summary of the Assay Procedure for Porcine Alpha-galactosyl ELISA kit

Citations of Porcine Alpha-galactosyl ELISA kit

E07A0482 has been referenced in the below publications:

Optimized decellularization protocol including a-Gal epitope reduction for fabrication of an acellular porcine annulus fibrosus scaffold.

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