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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Bcl 2 like protein 1 ELISA kit

  • Cell Biology
  • Cancer
  • Metabolism

E12B0764 Chicken Bcl 2 like protein 1 ELISA kit

Chicken Bcl 2 like protein 1 ELISA kit is suitable for the detection of samples from chicken species. Bcl 2 like protein 1 can also be called BCL2L1, Bcl2l1,Bcl(X)L, Bcl-XL, Bcl2l, BclX, bcl-x, bcl2-L-1, BCL-XL/S, BCLXL, BCLXS, PPP1R52, bcl-xS, BCL2L, BCLX, Bcl-X, bcl-xL, BCL2 like 1.

Specifications of Chicken Bcl 2 like protein 1 ELISA kit

Product Information

Cat. No.

E12B0764

Product Name

Chicken Bcl 2 like protein 1 (BCL2L1)ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

BCL2L1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BCL2L1 antibody and a BCL2L1-HRP conjugate. The assay sample and buffer are incubated together with BCL2L1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL2L1 concentration since BCL2L1 from samples and BCL2L1-HRP conjugate compete for the anti-BCL2L1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL2L1 from the sample, fewer sites are left to bind BCL2L1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABCL2L1 concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Bcl 2 like protein 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

86-115.87%

Linearity

Diluent Ratio

Range %

1:2

97-117

1:4

94-104

1:8

86 -101

Specificity/Cross-reactivity

No significant cross-reactivity or interference between BCL2L1 and analogues was observed.


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Summary of the Assay Procedure for Chicken Bcl 2 like protein 1 ELISA kit

Summary of the Assay Procedure for Chicken Bcl 2 like protein 1 ELISA kit

Citations of Chicken Bcl 2 like protein 1 ELISA kit

E12B0764 has been referenced in the below publications:

Roles of miRNA and MDV oncogene Meq in the transformation of chicken Marek's disease lymphoma.

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