Chicken Bcl 2 like protein 1 (BCL2L1) ELISA kit is suitable for the detection of samples from chicken species. Bcl 2 like protein 1 can also be called as BCL2L1; Bcl2l1;Bcl(X)L; Bcl-XL; Bcl2l; BclX; bcl-x; bcl2-L-1; BCL-XL/S; BCLXL; BCLXS; PPP1R52; bcl-xS; BCL2L; BCLX; Bcl-X; bcl-xL; BCL2 like 1.
Specifications Of E12B0764 Chicken BCL2L1 ELISA Kit
Porduct's Information | |
Cat.NO. | E12B0764 |
Porduct's Name | Chicken Bcl 2 like protein 1 (BCL2L1)ELISA kit |
Species | Chicken |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/ml |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
BCL2L1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-BCL2L1 antibody and a BCL2L1-HRP conjugate. The assay sample and buffer are incubated together with BCL2L1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL2L1 concentration since BCL2L1 from samples and BCL2L1-HRP conjugate compete for the anti-BCL2L1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL2L1 from the sample, fewer sites are left to bind BCL2L1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABCL2L1 concentration in each sample is interpolated from this standard curve. |
Quality Control On Chicken BCL2L1 ELISA Kit
CV | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery% | 86-115.87 | |
Linearity | Diluent ratio | Range % |
1:2 | 97-117 | |
1:4 | 94-104 | |
1:8 | 86 -101 | |
Specificity/ Cross-reactivity | No significant cross-reactivity or interference between BCL2L1 and analogues was observed. | |
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Summary Of The Assay Procedures For E12B0764 Chicken BCL2L1 ELISA Kit
Citations Of Chicken Bcl 2 like protein 1 (BCL2L1) ELISA Kit
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