E06I0036 Goat Interleukin 13 ELISA kit
Goat Interleukin 13 ELISA kit is suitable for the detection of samples from goat species. Interleukin 13 can also be called ALRH, BHR1, IL13, Interleukin13, Interleukin-13, IL 13, IL-13.
Specifications of Goat Interleukin 13 ELISA kit
Product Information | |
Cat. No. | E06I0036 |
Product Name | Goat Interleukin 13 ELISA kit |
Species | Goat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL 13 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 13 antibody and a IL 13-HRP conjugate. The assay sample and buffer are incubated together with IL 13-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 13 concentration since IL 13 from samples and IL 13-HRP conjugate compete for the anti- IL 13 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 13 from the sample, fewer sites are left to bind IL 13-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 13 concentration in each sample is interpolated from this standard curve. |
Quality Control on Goat Interleukin 13 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 87-116.86% | |
Linearity | Diluent Ratio | Range % |
1:2 | 92-111 | |
1:4 | 88-106 | |
1:8 | 85-102 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL 13 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Goat Interleukin 13 ELISA kit
Citations of Goat Interleukin 13 ELISA kit
E06I0036 has been referenced in the below publications:
Immune response against subclinical haemonchosis in Himalayan hill goats.
