Name: Hela & Human Host Cell DNA Residue Detection Kit
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Hela & Human Host Cell DNA Residue Detection Kit

HL-D100T Hela & Human Host Cell DNA Residue Detection Kit

The Hela & Human Host Cell DNA Residue Detection Kit can be used for quantitative analysis of DNA residue in recombinant protein expressed products, purified intermediate, and finished products from the host cell.

Products

Specifications of Hela & Human Host Cell DNA Residue Detection Kit

Introduction

This kit utilizes the TaqMan probe-based fluorescence quantitative PCR (qPCR) method. By employing highly specific primers and probes, it offers exceptional specificity and sensitivity, with a limit of quantification (LOQ) as low as 30 fg/μL. The kit is fully compatible with our Magnetic Residual DNA Sample Preparation Kit (Cat# CG-DP050 or CG-DP100), enabling seamless integration into your workflow.

The preparation process of DNA Control is completely consistent with that of the National Standard reference of host cell DNA y; therefore, it has high purity and no protein or ion interference. DNA Control has been calibrated by National Standard to ensure the accuracy of the sample quantitative detection.

The kit provides DNA Dilution Buffer, which enables good replicate parallelism in a single experiment and good reproducibility between multiple experiments.

Kit Components
DNA Amplification
Components NO.	Components Name	Cat#/Size HP-D050T(50T)
Components NO.	Components Name	Cat#/Size HP-D050T(50T)
B1	2XqPCR Mix	1.25mL
B2	Primer&Probe Mix	200μL
B3	DNA Dilution Buffer	4×1.5mL
B4	DNA Control (30ng/μL)	50μL
B5	RNase-Free H2O	1mL
B6	50X ROX Reference Dye(Optional)	0.3ml

Shipping and Storage
1	All components are shipped with ice bags.
2	The kit should be stored at -20℃ and it is recommended to be used within one year. The component of B2 should be stored protected from light.

PCR Reaction System on Hela Host Cell DNA Residue Detection Kit

Components	Volume(μL)
2XqPCR Mix	12.5
Primer&Probe Mix	2
DNA template (control or sample)	5
Add water	5.5
Total Volume	25

Mix solution = (number of reaction wells+4) * (12.5+2+5.5) μL (including the volume lost in the 4 wells).

The detection range of the standard curve mentioned above is suitable for most experiments and can be adjusted as needed.

Criteria for Results

Standard Curve: R²> 0.99; Amplification Efficiency: 90%≤Eff%≤110%; Slope: -3.8~-3.1.
The recovery rate of spiked samples=(measured value of spiked samples - measured value of samples)/theoretical value of spiked samples * 100%, with a range of 50% -150%.
No Template Control (NTC): In the reaction system, replacing the target template with DNA Dilution Buffer while keeping other components unchanged, and the Ct value obtained should be 'Undetermined' or detection concentration ≤ 5 fg/μL.