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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat soluble guanylate cyclase (E02S0462)

E02S0462 Rat soluble guanylate cyclase

The Rat soluble guanylate cyclase can be used to identify samples from the rat species. soluble guanylate cyclase can also be called sGC.

Products

Specifications of Rat soluble guanylate cyclase

Product Information

Cat. No.

E02S0462

Product Name

Rat soluble guanylate cyclase

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

sGC ELISA kit uses an anti-sGC antibody and an sGC-HRP conjugate in a competitive enzyme immunoassay method. sGC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-sGC antibody binding site between sGC from samples and sGC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of sGC. Since the number of sites is limited, as more sites are occupied by sGC from the sample, fewer sites are left to bind sGC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sGC concentration in each sample is interpolated from this standard curve.


Quality Control on Rat soluble guanylate cyclase

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between sGC and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat soluble guanylate cyclase

Summary of the Assay Procedure for Rat soluble guanylate cyclase

Citations of Rat soluble guanylate cyclase

E02S0462 has been referenced in the below publications:

Vasodilatory effect of formaldehyde via the NO/cGMP pathway and the regulation of expression of KATP, BKCa and L-type Ca2+ channels.


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