E02S0462 Rat soluble guanylate cyclase
The Rat soluble guanylate cyclase can be used to identify samples from the rat species. soluble guanylate cyclase can also be called sGC.
Specifications of Rat soluble guanylate cyclase
Product Information | |
Cat. No. | E02S0462 |
Product Name | Rat soluble guanylate cyclase |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
sGC ELISA kit uses an anti-sGC antibody and an sGC-HRP conjugate in a competitive enzyme immunoassay method. sGC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-sGC antibody binding site between sGC from samples and sGC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of sGC. Since the number of sites is limited, as more sites are occupied by sGC from the sample, fewer sites are left to bind sGC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sGC concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat soluble guanylate cyclase
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between sGC and analogues was observed. | |
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Summary of the Assay Procedure for Rat soluble guanylate cyclase
Citations of Rat soluble guanylate cyclase
E02S0462 has been referenced in the below publications：
Vasodilatory effect of formaldehyde via the NO/cGMP pathway and the regulation of expression of KATP, BKCa and L-type Ca2+ channels.
