E02I0010 Rat Interleukin 1β ELISA kit
The Rat Interleukin 1β ELISA kit can be used to identify samples from the rat species. Interleukin 1β can also be called Catabolin, H1, IL-1β, Hematopoietin 1, IFN beta inducing factor, IL 1, IL 1 beta, IL 1B, IL1B, IL1F2, Interleukin 1 beta, Interleukin 1 beta precursor, LAF, OAF, Osteoclast activating factor, Preinterleukin beta, Pro interleukin 1 beta, IInterleukin-1 beta, IL-1b, IL1b, IL 1b, IL-1β, IL 1β, IL1β.
Product Information | |
Cat. No. | E02I0010 |
Product Name | Rat Interleukin 1β ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IL 1β ELISA kit uses an anti-IL 1β antibody and an IL 1β-HRP conjugate in a competitive enzyme immunoassay method. IL 1β-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL 1β antibody binding site between IL 1β from samples and IL 1β-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL 1β. Since the number of sites is limited, as more sites are occupied by IL 1β from the sample, fewer sites are left to bind IL 1β-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 1β concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IL 1β and analogues was observed. | |
E02I0010 has been referenced in the below publications：
Therapeutic Effect of TSG-6 Engineered iPSC-Derived MSCs on Experimental Periodontitis in Rats: A Pilot Study.
Effects of chronic noise on glucose metabolism and gut microbiota-host inflammatory homeostasis in rats.
The Mechanisms Underlying the Antidepressant Effect of Acupuncture via Regulating the Immune/Inflammatory Response Mediated by TLR4 Signaling Pathway/NLRP3 Inflammasome in Rats Exposed to Chronic Restraint Stress.
The research of the role of the p38MAPK in the acute lung injury induced by PO in rats.
Progesterone protects neurons against impairment induced by Aβ activated astrocytes.
Protective Effect of Shenkang Injection Against Rats with Septic Acute Kidney Injury.
Untargeted Metabolomic Analysis of the Effects and Mechanism of Nuciferine Treatment on Rats With Nonalcoholic Fatty Liver Disease.
Mechanisms of Anti-inflammatory Property of Conserved Dopamine Neurotrophic Factor: Inhibition of JNK Signaling in Lipopolysaccharide-Induced Microglia.
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