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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Interleukin 1β ELISA kit (E02I0010)

E02I0010 Rat Interleukin 1β ELISA kit

The Rat Interleukin 1β ELISA kit can be used to identify samples from the rat species. Interleukin 1β can also be called Catabolin, H1, IL-1β, Hematopoietin 1, IFN beta inducing factor, IL 1, IL 1 beta, IL 1B, IL1B, IL1F2, Interleukin 1 beta, Interleukin 1 beta precursor, LAF, OAF, Osteoclast activating factor, Preinterleukin beta, Pro interleukin 1 beta, IInterleukin-1 beta, IL-1b, IL1b, IL 1b, IL-1β, IL 1β, IL1β.

Products

Specifications of Rat Interleukin 1β ELISA kit

Product Information

Cat. No.

E02I0010

Product Name

Rat Interleukin 1β ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL 1β ELISA kit uses an anti-IL 1β antibody and an IL 1β-HRP conjugate in a competitive enzyme immunoassay method. IL 1β-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL 1β antibody binding site between IL 1β from samples and IL 1β-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL 1β. Since the number of sites is limited, as more sites are occupied by IL 1β from the sample, fewer sites are left to bind IL 1β-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 1β concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Interleukin 1β ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL 1β and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Interleukin 1β ELISA kit

Summary of the Assay Procedure for Rat Interleukin 1β ELISA kit

Citations of Rat Interleukin 1β ELISA kit

E02I0010 has been referenced in the below publications:

Therapeutic Effect of TSG-6 Engineered iPSC-Derived MSCs on Experimental Periodontitis in Rats: A Pilot Study.

Effects of chronic noise on glucose metabolism and gut microbiota-host inflammatory homeostasis in rats.

The Mechanisms Underlying the Antidepressant Effect of Acupuncture via Regulating the Immune/Inflammatory Response Mediated by TLR4 Signaling Pathway/NLRP3 Inflammasome in Rats Exposed to Chronic Restraint Stress.

The research of the role of the p38MAPK in the acute lung injury induced by PO in rats.

Progesterone protects neurons against impairment induced by Aβ activated astrocytes.

Protective Effect of Shenkang Injection Against Rats with Septic Acute Kidney Injury.

Untargeted Metabolomic Analysis of the Effects and Mechanism of Nuciferine Treatment on Rats With Nonalcoholic Fatty Liver Disease.

Mechanisms of Anti-inflammatory Property of Conserved Dopamine Neurotrophic Factor: Inhibition of JNK Signaling in Lipopolysaccharide-Induced Microglia.


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