E02N0086 Rat N MID Osteocalcin ELISA kit
The Rat N MID Osteocalcin ELISA kit can be used to identify samples from the rat species. N MID Osteocalcin can also be called N MID OT.
Specifications of Rat N MID Osteocalcin ELISA kit
Product Information | |
Cat. No. | E02N0086 |
Product Name | Rat N MID Osteocalcin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/mL |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
N MID OT ELISA kit uses an anti-N MID OT antibody and an N MID OT-HRP conjugate in a competitive enzyme immunoassay method. N MID OT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-N MID OT antibody binding site between N MID OT from samples and N MID OT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of N MID OT. Since the number of sites is limited, as more sites are occupied by N MID OT from the sample, fewer sites are left to bind N MID OT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The N MID OT concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat N MID Osteocalcin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between N MID OT and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat N MID Osteocalcin ELISA kit
Citations of Rat N MID Osteocalcin ELISA kit
E02N0086 has been referenced in the below publications：
Sclerostin antibody and interval treadmill training effects in a rodent model of glucocorticoid-induced osteopenia.
