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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Anti double stranded DNA ELISA kit

  • Epigenetics and Nuclear Signaling

E01D0004 Human Anti double stranded DNA ELISA kit

The Human Anti double stranded DNA ELISA kit can be used to identify samples from the human species. Anti double stranded DNA can also be called anti dsDNA, dsDNA-Ab.

Specifications of Human Anti double stranded DNA ELISA kit

Product Information

Cat. No.

E01D0004

Product Name

Human Anti double stranded DNA ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

25-500 IU/mL

Sensitivity

1.0 IU/mL

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 IU/mL

1 vial

STANDARD B (0.5mL)

25 IU/mL

1 vial

STANDARD C (0.5mL)

50 IU/mL

1 vial

STANDARD D (0.5mL)

100 IU/mL

1 vial

STANDARD E (0.5mL)

250 IU/mL

1 vial

STANDARD F (0.5mL)

500 IU/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Anti-dsDNA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with dsDNA antigen. Standards or samples are then added to the microtiter plate wells and anti-dsDNA if present, will bind to the antigen pre-coated wells. In order to quantitatively determine the amount of anti-dsDNA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated anti-dsDNA antibody IgG is added to each well to “sandwich” the anti-dsDNA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Anti-dsDNA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Anti-dsDNA concentration in each sample is interpolated from this standard curve.


Quality Control on Human Anti double stranded DNA ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between anti dsDNA and analogues was observed.


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Summary of the Assay Procedure for Human Anti double stranded DNA ELISA kit

Summary of the Assay Procedure for Human Anti double stranded DNA ELISA kit

Citations of Human Anti double stranded DNA ELISA kit

E01D0004 has been referenced in the below publications:

DAMP production by human islets under low oxygen and nutrients in the presence or absence of an immunoisolating-capsule and necrostatin-1.

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