E01P0023 Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit
The Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit can be used to identify samples from the human species. Phosphorylation P38 Mitogen activated Protein Kinase can also be called P P38MAPK.
Product Information | |
Cat. No. | E01P0023 |
Product Name | Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 25 ng/mL | 1 vial |
STANDARD C (0.5mL) | 50 ng/mL | 1 vial |
STANDARD D (0.5mL) | 100 ng/mL | 1 vial |
STANDARD E (0.5mL) | 250 ng/mL | 1 vial |
STANDARD F (0.5mL) | 500 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
P P38MAPK ELISA kit uses an anti-P P38MAPK antibody and an P P38MAPK-HRP conjugate in a competitive enzyme immunoassay method. P P38MAPK-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-P P38MAPK antibody binding site between P P38MAPK from samples and P P38MAPK-HRP conjugate, the intensity of the color is inversely proportional to the concentration of P P38MAPK. Since the number of sites is limited, as more sites are occupied by P P38MAPK from the sample, fewer sites are left to bind P P38MAPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P P38MAPK concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between P P38MAPK and analogues was observed. | |
E01P0023 has been referenced in the below publications：
Dissertation Submitted to Guangzhou Sport University for Master Degree.
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