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BlueGene Biotech Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit (E01P0023)

E01P0023 Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit

The Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit can be used to identify samples from the human species. Phosphorylation P38 Mitogen activated Protein Kinase can also be called P P38MAPK.

Products

Specifications of Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit

Product Information

Cat. No.

E01P0023

Product Name

Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

25-500 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

P P38MAPK ELISA kit uses an anti-P P38MAPK antibody and an P P38MAPK-HRP conjugate in a competitive enzyme immunoassay method. P P38MAPK-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-P P38MAPK antibody binding site between P P38MAPK from samples and P P38MAPK-HRP conjugate, the intensity of the color is inversely proportional to the concentration of P P38MAPK. Since the number of sites is limited, as more sites are occupied by P P38MAPK from the sample, fewer sites are left to bind P P38MAPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P P38MAPK concentration in each sample is interpolated from this standard curve.


Quality Control on Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between P P38MAPK and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit

Summary of the Assay Procedure for Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit

Citations of Human Phosphorylation P38 Mitogen activated Protein Kinase ELISA kit

E01P0023 has been referenced in the below publications:

Dissertation Submitted to Guangzhou Sport University for Master Degree.

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