Human Protein Induced by Vitamine k absence or antagonist II ELISA kit

BlueGene Biotech Human Protein Induced by Vitamine k absence or antagonist II ELISA kit

E01P1008 Human Protein Induced by Vitamine k absence or antagonist II ELISA kit

The Human Protein Induced by Vitamine k absence or antagonist II ELISA kit can be used to identify samples from the human species. Protein Induced by Vitamine k absence or antagonist II can also be called PIVKA-II, PIVKA2, DCP, antagonist-II, Des-gamma carboxyprothrombin.

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Specifications of Human Protein Induced by Vitamine k absence or antagonist II ELISA kit

Product Information
Cat. No.	E01P1008
Product Name	Human Protein Induced by Vitamine k absence or antagonist II ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	1.0-25 ng/ml
Sensitivity	0.1 ng/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	1.0 ng/mL	1 vial
STANDARD C (0.5mL)	2.5 ng/mL	1 vial
STANDARD D (0.5mL)	5.0 ng/mL	1 vial
STANDARD E (0.5mL)	10 ng/mL	1 vial
STANDARD F (0.5mL)	25 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

PIVKA-II ELISA kit uses an anti-PIVKA-II antibody and an PIVKA-II-HRP conjugate in a competitive enzyme immunoassay method. PIVKA-II-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PIVKA-II antibody binding site between PIVKA-II from samples and PIVKA-II-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PIVKA-II. Since the number of sites is limited, as more sites are occupied by PIVKA-II from the sample, fewer sites are left to bind PIVKA-II-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PIVKA-II concentration in each sample is interpolated from this standard curve.

Quality Control on Human Protein Induced by Vitamine k absence or antagonist II ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between PIVKA-II and analogues was observed.

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Summary of the Assay Procedure for Human Protein Induced by Vitamine k absence or antagonist II ELISA kit

Citations of Human Protein Induced by Vitamine k absence or antagonist II ELISA kit

E01P1008 has been referenced in the below publications：

Comparing Prothrombin induced by vitamin K absence-II (PIVKA-II) with the oncofetal proteins Glypican-3, Alpha feto protein and Carcinoembryonic antigen in diagnosing hepatocellular carcinoma among Egyptian patients.